Interferon- Induces Apoptosis of Lens TN4–1 Cells and Proteasome Inhibition Has an Antiapoptotic Effect
نویسندگان
چکیده
METHOD. Murine lens epithelial TN4-1 cells were treated with IFN. Apoptosis was measured using annexin V-FITC and propidium iodide (PI) staining, and DNA fragmentation. IFN–inducible mRNA and protein expressions were measured by RT-PCR and Western blot analysis. Caspase activities were measured using colorimetric substrates and poly (ADP ribose) polymerase (PARP) cleavage. The effect of proteasome inhibition was tested with MG132 and lactacystin.
منابع مشابه
Upregulation of heat shock protein expression by proteasome inhibition: an antiapoptotic mechanism in the lens.
PURPOSE Studies have shown that proteasome inhibition protects lens epithelial cells (LECs) against interferon (IFN)-gamma-induced apoptosis. The present study was conducted to test the hypothesis that proteasome inhibition can protect lens cells against apoptosis by upregulating heat shock protein (HSP) expression. METHODS Murine lens epithelial alphaTN4-1 cells were treated with combination...
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